james bertram wrote:But I am still a bit

Hi James,
then I'm afraid I don't really understand what the question is aiming at.
If the question is whether WUFI assumes or requires that the thermal conductivity to be used for a material has been determined with some specific measurement method, then the answer is: no, it doesn't.
The material parameters provided by the user should simply be "adequate for the task". Depending on the relevance of a particular material parameter for the simulation results, it may be sufficient to guess it, or to take it from published tables describing similar materials, or to measure it with crude and quick methods. If the result depends very sensitively on a certain parameter, it may be necessary to measure this parameter very carefully.
As to the thermal conductivity in particular, experience shows that water contents usually only depend weakly on the thermal conductivities of the materials in the assembly. It is then sufficient to simply use "reasonable" numbers.
If you are worrying whether a measured conductivity which is stated to be valid for a certain mean temperature should be adjusted for use in a simulation in which a different mean temperature prevails: this is usually not necessary. If you are not sure how sensitively a simulation result depends on a certain simulation parameter and how accurately this parameter needs to be determined, simply run a few test simulations with variations of this parameter. In most cases you will find that the simulation result only depends so weakly on these variations that your assessment of the simulation (e.g. construction fails / doesn't fail) would remain unaffected. If you find that varying a parameter does make a significant difference, you can use the test calculations to find out how accurately that parameter needs to be determined.
In the unlikely case that you find it necessary to adjust your conductivity to the mean temperature occurring in the simulation, you have to do this by hand and enter the result in WUFI.
Kind regards,
Thomas